Mito-Photo-DNP is a mitochondria-targeted, photo-activated protonophore1. This novel tool consists of three structural units, a caged protonophore, a photocleavable linker coupled to a triphenylphosphonium moiety which confers selective uptake by the mitochondria2. Cells are treated with Mito-Photo-DNP at 200 nM and irradiated with UV light at 355 nm. Mito-Photo-DNP can be employed for selective uncoupling of either individual or a small number of mitochondria within a cell when used with fluorescence imaging1,3.
1) Chalmers et al. (2012), Selective uncoupling of individual mitochondria within a cell using mitochondria-targeted photoactivated protonophore; J. Am. Chem.Soc., 134 758
2) Smith et al. (2012), Mitochondrial pharmacology; Trends Pharmacol. Sci., 33 341
3) Glancy et al. (2015), Mitochondrial reticulum for cellular energy distribution in muscle; Nature, 523 617