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Product Details

20(S)-Hydroxycholesterol | Smoothened activator

20-(S)-Hydroxycholesterol (516-72-3) is an endogenous ligand for the LXR receptor.1 It has anti-adipogenic and pro-osteogenic effects in mesenchymal stem cells mediated via a non-LXR dependent pathway.2 The osteogenic effects of 20-(S)-hydroxycholesterol have been shown to be mediated by activation of hedgehog signaling and expression of genes associated with Notch signaling.3 Activation of Hedgehog is mediated via binding of 20(S)-hydroxycholesterol to Smoothened at a site distinct from the cyclopamine binding site.4,5

1) Janowski et al. (1996), An oxysterol signaling pathway mediated by the nuclear receptor LXR7alpha;; Nature, 383 728
2) Kha et al. (2004), Oxysterols regulate differentiation of mesenchymal stem cells: Pro-bone and Anti-fat: J. Bone Min. Res., 19 830
3) Kim et al. (2010), Osteogenic oxysterol, 20(S)-hydroxycholesterol, induces notch target gene expression in bone marrow stromal cells; J. Bone Miner. Res., 25 7823
4) Dwyer et al. (2007), Oxysterols are novel activators of the hedgehog signaling pathway in pluripotent mesenchymal cells; J. Biol. Chem., 282 8959
5) Nedelcu et al. (2013); Oxysterol binding to the extracellular domain of Smoothened in Hedgehog signaling; Nat. Chem. Biol., 9 557

Focus Biomolecules reagent supplier, chemical structure of 20(S)-Hydroxycholesterol | Smoothened activator | CAS 516-72-3

Catalog#  10-4540

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Catalog Number:
Smoothened activator
Chemical Name:
5-Cholesten-3β, 20α-diol
Molecular Weight:
Molecular Formula:
Soluble in DMSO or in Ethanol.
Physical Properties:
White solid
98% by TLC
NMR (Conforms)
Storage Temperature:
Stable for 1 year as supplied. Solutions in DMSO or ethanol may be stored at -20° for up to 3 months.
Shipping Code:
Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications.

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