C6-NBD Ceramide | Fluorescent probe for the Golgi apparatus

C6-NBD Ceramide (94855-02-6) is a selective stain for the Golgi apparatus.1 Readily taken up by MDCK cells and converted into C6-NBD-sphingomyelin and C6-NBD-glucosyl-ceramide.2 A useful substrate for assay of ceramide kinase.3 May be used to monitor the biosynthesis and degradation of sphinogmyelin in cellular systems.4  Ex: 455 nm and Em: 533 nm.4

References:

  1. Chazotte (2012), Labeling Golgi with Fluorescent Ceramides; Cold Spring Harb. Protoc. (8):pdb.prot070599
  2. van Meer et al. (1987), Sorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells; J. Cell Biol. 105 1623
  3. Don and Rosen (2008), A fluorescent plate reader assay for ceramide kinase; Anal. Biochem. 375 265
  4. Nikolova-Karakashian (2021), Methods to Characterize Synthesis and Degradation of Sphingomyelin at the Plasma Membrane and Its Impact on Lipid Raft Dynamics; Methods Mol. Biol. 2187 113
Weight 0.1 mg
Dimensions 0.1 × 0.1 × 0.1 cm
Size:

1 mg, 5 mg

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CAS:
94885-02-6
Catalog Number:
10-5496
Activity:
Fluorescent probe for the Golgi apparatus
Chemical Names:
N-[7-(4-Nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine; N-(NBD-aminohexanoyl) D-erythro-sphingosine
Molecular Weight:
575.75
Molecular Formula:
C30H49N5O6
Solubility:
Soluble in DMSO (35 mg/mL)
Physical Properties:
Orange solid
Purity:
>98% TLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Stable for 2 years as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.
Shipping Code:
RT

C6-NBD Ceramide | Fluorescent probe for the Golgi apparatus

$100.00$400.00

CAS:
94885-02-6
Catalog Number:
10-5496
Activity:
Fluorescent probe for the Golgi apparatus
Chemical Names:
N-[7-(4-Nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine; N-(NBD-aminohexanoyl) D-erythro-sphingosine
Molecular Weight:
575.75
Molecular Formula:
C30H49N5O6
Solubility:
Soluble in DMSO (35 mg/mL)
Physical Properties:
Orange solid
Purity:
>98% TLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Stable for 2 years as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.
Shipping Code:
RT
  • 1 mg
  • 5 mg
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