Modify Search
Select all
Products
Pages
Posts
Modify Search
Select all
Products
Pages
Posts

5-Ethynyl-2′-deoxyuridine | DNA labeling

CAS:
61135-33-9
Catalog Number:
10-2794
Activity:
DNA labeling
Alternate Names:
5-EdU
Chemical Name:
2′-Deoxy-5-ethynyluridine
Molecular Weight:
252.23
Molecular Formula:
C11H12N2O5
Solubility:
Soluble in DMSO (up to 10 mg/ml).
Physical Properties:
White or off-white solid
Purity:
98% by HPLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 2 months.
Shipping Code:
RT

5-Ethynyl-2′-deoxyuridine (61135-33-9) is a novel agent for fast and sensitive detection of DNA synthesis in vivo.1 5-EdU is incorporated into proliferating cells followed by detection with a fluorescent azide using click ligation.1 In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. 5-Ethynyl-2′-deoxyuridine may be used for studying cell proliferation in the central nervous system and may be combined with BrdU staining in a novel protocol allowing for double labeling of DNA synthesis.2

References/Citations:

1) Salic and Mitchison (2008), A chemical method for fast and sensitive detection of DNA synthesis in vivo; Proc. Natl. Acad. Sci. USA, 105 2415
2) Zeng et al. (2010), Evaluation of 5-ethynyl-2′-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system; Brain Res., 1319C 21

5-Ethynyl-2′-deoxyuridine | DNA labeling is also available in the Cellular Metabolism Library.
CAS:
61135-33-9
Catalog Number:
10-2794
Activity:
DNA labeling
Alternate Names:
5-EdU
Chemical Name:
2′-Deoxy-5-ethynyluridine
Molecular Weight:
252.23
Molecular Formula:
C11H12N2O5
Solubility:
Soluble in DMSO (up to 10 mg/ml).
Physical Properties:
White or off-white solid
Purity:
98% by HPLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 2 months.
Shipping Code:
RT

Safety Data Sheet:

N/A

Product Data Sheet:

Add to Quote

Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications. Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a $20 restocking/refund fee