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Product Details

ML-323 | USP-1/UAF-1 inhibitor

ML-323 (1571424-83-5) is a potent and reversible USP1-UAF1 deubiquitinase complex inhibitor with excellent selectivity against human DUBs, deSUMOylase, deneddylase and unrelated proteases, IC50=76 nM in a ubiquitin-rhodamine 110 assay.1 In H1299 non-small cell lung cancer cells treatment with ML-323 dose dependently and robustly increased levels of monoubiquitinated PCNA compared to untreated cells at concentrations as low as 1 μM.1 It potentiates cisplatin cytotoxicity in non-small cell lung cancer and osteosarcoma cells.2  ML-323 inhibition of upregulated USP1 in BRCA1-deficient tumor cells results in replication fork destabilization and decreased viability.3


1) Dexheimer et al. (2014), Synthesis and structure-activity relationship studies of N-benzyl-2-phenylpyrimidin-4-amine derivatives as potent USP1/UAF1 deubiquitinase inhibitors with anticancer activity against nonsmall cell lung cancer; J. Med. Chem., 57 8099
2) Liang et al. (2014), A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses; Nat. Chem. Biol., 10 298
3) Lim et al. (2018) USP1 is required for replication fork protection in BRCA1-deficient tumors; Mol. Cell, 72 925

Focus Biomolecules cell signaling reagent supplier, chemical structure of ML-323 | USP-1/UAF-1 inhibitor | CAS 1572414-83-5

Catalog#  10-1560-0005

Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a $20 restocking/refund fee.
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Catalog Number:
USP-1/UAF-1 inhibitor
Chemical Name:
Alternate Names:
CID 60167849; NCGC00262306
Molecular Weight:
Molecular Formula:
Soluble in DMSO (up to 40 mg/ml), or in Ethanol (up to 20 mg/ml)
Physical Properties:
White solid
98% by TLC
NMR (Conforms)
Storage Temperature:
-20°C (des.)
Stable for 1 year as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 3 months.
Shipping Code:
Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications.

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