Biotin isoxazole | Precipitates RNA Binding Proteins

Biotin-isoxazole (1377605-22-5) precipitates hundreds of RNA-binding proteins with significant overlap to the constituents of RNA granules.1,2 In one study roughly 580 nuclear proteins were identified as being precipitated by B-Isox including TAF15, RNA polymerase II (largest subunit), numerous subunits of the mediator complex as well as a variety of enzymes involved in epigenetic modification of histones.3  SR domains were found to be the determinant that facilitates B-Isox precipitation.4 Also allows for the fractionation / isolation of SG-associated proteins and RNA precipitates.5

References:

  1. Kato et al. (2012), Cell-free formation of rNA granules: low complexity sequence domains form dynamic fibers within hydrogels; Cell, 149 753
  2. Han et al. (2012), Cell-free formation of RNA granules: bound RNAs identify features and components of cellular assemblies; Cell, 149 768
  3. Kwon et al. (2013), Phosphorylation-regulated binding of RNA polymerase II to fibuour polymers of low-complexity domains; Cell, 155 1049
  4. Kwon et al. (2014), Poly-dipeptides encoded by the C9orf72 repeats bind nucleoli, impede RNA biogenesis, and kill cells; Science, 345 1139
  5. Panas et al. (2015), Methods for the characterization of stress granules in virus infected cells; Methods, 90 57
Weight 0.1 mg
Dimensions 0.1 × 0.1 × 0.1 cm
Size:

1 mg, 5 mg

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CAS:
1377605-22-5
Catalog Number:
10-5400
Activity:
Precipitates RNA binding proteins
Chemical Names:
6-(5-(Thiophen-2-yl)isoxazole-3-carboxamido)hexyl 5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate
Molecular Weight:
520.66
Molecular Formula:
C24H32N4O5S2
Solubility:
Soluble in DMSO (30 mg/ml with warming)
Physical Properties:
Beige solid
Purity:
98% TLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Stable for 2 years as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.
Shipping Code:
RT

Biotin isoxazole | Precipitates RNA Binding Proteins

$100.00$360.00

CAS:
1377605-22-5
Catalog Number:
10-5400
Activity:
Precipitates RNA binding proteins
Chemical Names:
6-(5-(Thiophen-2-yl)isoxazole-3-carboxamido)hexyl 5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate
Molecular Weight:
520.66
Molecular Formula:
C24H32N4O5S2
Solubility:
Soluble in DMSO (30 mg/ml with warming)
Physical Properties:
Beige solid
Purity:
98% TLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Stable for 2 years as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.
Shipping Code:
RT
  • 1 mg
  • 5 mg
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