DAPI | Fluorescent DNA stain

DAPI (28718-90-3) is a fluorescent DNA stain for use on fixed cells for applications such as imaging, mitochondrial DNA detection1, or nuclear quantitation2. This dye, often used as a counterstain in immunofluorescence, binds strongly to AT rich runs of nucleic acid, causing its fluorescent emission to increase.3 Ex 358 nm; Em 461 nm (dsDNA), Em 500 (RNA).

  1. Russell et al. (1975), A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses; Nature, 253 461
  2. Tarnowski et al. (1991), DAPI as a useful stain for nuclear quantitation; Biotech Histochem., 66 297
  3. Estandarte et al. (2016), The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes; Rep., 6 31417
Weight 0.01 mg
Dimensions 0.01 × 0.01 × 0.01 cm
Size:

5 mg, 25 mg

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CAS:
28718-90-3
Catalog Number:
10-5065
Activity:
Fluorescent DNA stain
Chemical Name:

4′,6-Diamidino-2-phenylindole, dihydrochloride

Molecular Weight:
350.25
Molecular Formula:
C16H15N5·2HCl
Solubility:
Soluble in DMSO (up to 35 mg/ml) or water (up to 20 mg/ml with warming)
Physical Properties:
Yellow solid
Purity:
98% by TLC/HPLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
PROTECT FROM LIGHT! Stable for 2 years as supplied. Solutions in DMSO may be aliquoted and stored at -20°C for up to 3 months.
Shipping Code:
RT

DAPI | Fluorescent DNA stain

$40.00$130.00

CAS:
28718-90-3
Catalog Number:
10-5065
Activity:
Fluorescent DNA stain
Chemical Name:

4′,6-Diamidino-2-phenylindole, dihydrochloride

Molecular Weight:
350.25
Molecular Formula:
C16H15N5·2HCl
Solubility:
Soluble in DMSO (up to 35 mg/ml) or water (up to 20 mg/ml with warming)
Physical Properties:
Yellow solid
Purity:
98% by TLC/HPLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
PROTECT FROM LIGHT! Stable for 2 years as supplied. Solutions in DMSO may be aliquoted and stored at -20°C for up to 3 months.
Shipping Code:
RT
  • 5 mg
  • 25 mg
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SKU: 10-5065 Categories: ,