DAPI (28718-90-3) is a fluorescent DNA stain for use on fixed cells for applications such as imaging, mitochondrial DNA detection1, or nuclear quantitation2. This dye, often used as a counterstain in immunofluorescence, binds strongly to AT rich runs of nucleic acid, causing its fluorescent emission to increase.3 Ex 358 nm; Em 461 nm (dsDNA), Em 500 (RNA).
- Russell et al. (1975), A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses; Nature, 253 461
- Tarnowski et al. (1991), DAPI as a useful stain for nuclear quantitation; Biotech Histochem., 66 297
- Estandarte et al. (2016), The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes; Rep., 6 31417