Geldanamycin-FITC is a novel geldanamycin fluorescent probe¹ that may be used in a fluorescence polarization assay for HSP-90 inhibitors.^2,3 May also be used for detection of cell surface HSP-90 and for other applications. Fluorescent geldanamycin probes have been used to characterize geldanamycin drug-target interactions for effective translation of in vitro pharmacology to cellular and in vivo efficacy.⁴

References/Citations

1) Llauger-Bufi et al. (2003), Synthesis of novel fluorescent probes for the molecular chaperone Hsp90;  Bioorg. Med. Chem. Lett., 13 3975
2) Kim et al. (2004), Development of a fluorescent polarization assay for the molecular chaperone Hsp90;  J. Biomol. Screen., 9 375
3) Austin et al. (2012), Fragment screening using capillary electrophoresis (Defrag) for hit identification of heat shock protein 90 ATPase inhibitors;  J. Biomol. Screen., 17 868
4) Gooljarsingh et al. (2006), A biochemical rationale for the anticancer effects of Hsp90 inhibitors: slow, tight binding inhibition by geldanamycin and its analogues;  Proc. Natl. Acad. Sci. USA, 103 7625