4-MUG | ß-Galactosidase fluorogenic substrate

4-MUG (6160-78-7) is a versatile fluorogenic substrate for ß-galactosidase¹ for use in cell extracts or purified enzyme preparations.^1-3  May be used as a fluorescent probe for identification of senescent cells based on lysosomal ß-galactosidase activity.^4,5 This substrate produces a water soluble blue fluorescent coumarin fluorophore. Ex: 342 nm; Em: 441 nm

References:

1. Chiu et al. (2017), Measuring ß-Galactosidase Activity in Gram-Positive Bacteria Using a Whole-Cell Assay with MUG as a Fluorescent Reporter; Curr. Protoc. Toxicol., Supp 74 4.44.1
2. Kytidou et al. (2018), Nicotiana benthamiana α-galactosidase A1,1 can functionally complement human α-galactosidase A deficiency associated with Fabry disease; J. Biol. Chem., 293 10042
3. Hernandez-Guzman et al. (2016), Purification and characterization of an extracellular ß-glucosidase from Sporothrix schenckii; FEBS Open Bio., 6 1067
4. Lee et al. (2006),  Senescence-associated beta-galactosidase is lysosomal beta-galactosidase; Aging Cell, 5 187
5. Sosinska et al. (2014), Specificity of cytochemical and fluorescence methods of senescence-associated ß-galactosidase detection for ageing driven by replication and time,  Biogerontology, 15 407