5-Ethynyl-2′-deoxyuridine | DNA labeling
NMR (Conforms)
Available Options
| Size : | Price | Quantity | |
|---|---|---|---|
| 50 mg | $40.00 | ||
| 250 mg | $150.00 |
5-Ethynyl-2′-deoxyuridine (61135-33-9) is a novel agent for fast and sensitive detection of DNA synthesis in vivo.1 5-EdU is incorporated into proliferating cells followed by detection with a fluorescent azide using click ligation.1 In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. 5-Ethynyl-2′-deoxyuridine may be used for studying cell proliferation in the central nervous system and may be combined with BrdU staining in a novel protocol allowing for double labeling of DNA synthesis.2
References/Citations:
1) Salic and Mitchison (2008), A chemical method for fast and sensitive detection of DNA synthesis in vivo; Proc. Natl. Acad. Sci. USA, 105 2415
2) Zeng et al. (2010), Evaluation of 5-ethynyl-2′-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system; Brain Res., 1319C 21
NMR (Conforms)
Safety Data Sheet:
Product Data Sheet:
Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications. Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a $20 restocking/refund fee
5-Ethynyl-2′-deoxyuridine (61135-33-9) is a novel agent for fast and sensitive detection of DNA synthesis in vivo.1 5-EdU is incorporated into proliferating cells followed by detection with a fluorescent azide using click ligation.1 In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. 5-Ethynyl-2′-deoxyuridine may be used for studying cell proliferation in the central nervous system and may be combined with BrdU staining in a novel protocol allowing for double labeling of DNA synthesis.2
References/Citations:
1) Salic and Mitchison (2008), A chemical method for fast and sensitive detection of DNA synthesis in vivo; Proc. Natl. Acad. Sci. USA, 105 2415
2) Zeng et al. (2010), Evaluation of 5-ethynyl-2′-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system; Brain Res., 1319C 21
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