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5-Ethynyl-2'-deoxyuridine | DNA labeling

5-Ethynyl-2'-deoxyuridine (61135-33-9) is a novel agent for fast and sensitive detection of DNA synthesis in vivo1. 5-EdU is incorporated into proliferating cells followed by detection with a fluorescent azide using click ligation1. In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. 5-Ethynyl-2'-deoxyuridine may be used for studying cell proliferation in the central nervous system and may be combined with BrdU staining in a novel protocol allowing for double labeling of DNA synthesis2.

References/Citations
1) Salic and Mitchison (2008), A chemical method for fast and sensitive detection of DNA synthesis in vivo; Proc. Natl. Acad. Sci. USA, 105 2415
2) Zeng et al. (2010), Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system; Brain Res., 1319C 21

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Catalog#  10-2794

$50.00
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Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a $20 restocking/refund fee.
 
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CAS:
61135-33-9
Catalog Number:
10-2794
Activity:
DNA labeling
Alternate Names:
5-EdU
Chemical Name:
2'-Deoxy-5-ethynyluridine
Molecular Weight:
252.23
Molecular Formula:
C11H12N2O5
Solubility:
Soluble in DMSO (up to 10 mg/ml).
Physical Properties:
White or off-white solid
Purity:
98% by HPLC
NMR (Conforms)
Storage Temperature:
-20°C (des.)
Stability:
Stable for 1 year as supplied. Solutions in DMSO may be stored at -20° for up to 2 months.
Shipping Code:
RT
Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications.

Focus BiomoleculesPlymouth Meeting, PA USA1-855-FOCUS21

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