Antipain 2HCl | Serine and cysteine protease inhibitor

Antipain 2HCl (CAS 37682-72-7) is a serine/cysteine protease inhibitor. Also inhibits some trypsin-like proteases.¹ May be used as an additive to prevent proteolytic activity during recombinant protein production in Sf-9 insect cell cultures.² May be employed in the characterization of newly discovered proteases.^3,4 Blocks autophagy in cultured tobacco cells and may be used in an assay for autophagy in plant cells.⁵

References/Citations:

1) Umezawa et al. (1976), Structures and activities of protease inhibitors of microbial origin; Method Enzymol., 45 678
2) Gotoh et al. (2001), Proteolytic activity and recombinant protein production in virus-infected Sf-9 insect cell cultures supplemented with carboxyl and cysteine protease inhibitors; J. Biosci. Bioeng., 92 248
3) Hockensmith et al. (2016), Identification and characterization of a chymotrypsin-like serine protease from periodontal pathogen, Tannerella forsythia; Microb. Pathog., 100 37
4) Mat Amin et al. (2004), Proteinases in Naegleria Fowleri (strain NF3), a pathogenic amoeba: a preliminary study.; Trop. Biomed., 21 57
5) Moriyyasu & Inoue (2008), Use of protease inhibitor for detecting autophagy in plants; Methods Enzymol., 451 557