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Biotinyl tyramide | Signal amplification reagent for immunoassays

Reagent for use in catalyzed reporter deposition (CARD) signal amplification protocols in a variety of immunoassays in which, horseradish peroxidase catalyzed deposition of biotinyl tyramide is detected with labeled streptavidin.1 Widely used for signal amplification in fluorescent in situ hybridization (FISH) protocols.2 May be used for signal amplification in a sandwich ELISA for quantification of α-tubulin isotypes.3 Reagent for “self-biotinylation” of DNA G-quadruplexes.4 May be used with APEX-mediated biotin labeling to identify protein-protein interactions.5


1) Bobrow et al. (1989), Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays; J. Immunol. Methods, 125 279
2) Evans et al. (2003), Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications in formalin-fixed, paraffin-embedded tissue specimens; BMC. Clin. Pathol., 3 2
3) Draerova et al. (2013), Quantification of a-tubulin by sandwich ELISA with signal amplification through niotinyl-tyramide or immune-PCR; J. Immunol. Methods, 395 63
4) Einarson and Sen (2017), Self-biotinylation of DNA G-quadruplexes via intrinsic peroxidase activity; Nucleic Acids Res., 45 9813
5) Hwang and Espenshade (2016), Proximity-dependent biotinlabelling in yeast using the engineered ascorbate peroxidase APEX2; Biochem. J., 473 2463

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Catalog#  10-3446-0020

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Catalog Number:
Signal amplification reagent for immunoassays
Chemical Name:
Alternate Names:
Molecular Weight:
Molecular Formula:
Soluble in DMSO (up to 35 mg/ml) or in Ethanol (up to 20 mg/ml)
Physical Properties:
White solid
98% by HPLC
NMR (Conforms)
Storage Temperature:
-20°C (des.)
Stable for 1 year as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month.
Shipping Code:
Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications.

Focus BiomoleculesPlymouth Meeting, PA USA1-855-FOCUS21

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