C6-NBD Ceramide | Fluorescent probe for the Golgi apparatus

CAS:
94885-02-6
Catalog Number:
10-5496
Activity:
Fluorescent probe for the Golgi apparatus
Chemical Names:
N-[7-(4-Nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine; N-(NBD-aminohexanoyl) D-erythro-sphingosine
Molecular Weight:
575.75
Molecular Formula:
C30H49N5O6
Solubility:
Soluble in DMSO (35 mg/mL)
Physical Properties:
Orange solid
Purity:
>98% TLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Stable for 2 years as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.
Shipping Code:
RT

Available Options

Size:PriceQuantity 
1 mg$100.00
5 mg$400.00

C6-NBD Ceramide (94885-02-6) is a selective stain for the Golgi apparatus.1 Readily taken up by MDCK cells and converted into C6-NBD-sphingomyelin and C6-NBD-glucosyl-ceramide.2 A useful substrate for assay of ceramide kinase.3 May be used to monitor the biosynthesis and degradation of sphinogmyelin in cellular systems.4  Ex: 455 nm and Em: 533 nm.4

References:

  1. Chazotte (2012), Labeling Golgi with Fluorescent Ceramides; Cold Spring Harb. Protoc. (8):pdb.prot070599
  2. van Meer et al. (1987), Sorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells; J. Cell Biol. 105 1623
  3. Don and Rosen (2008), A fluorescent plate reader assay for ceramide kinase; Anal. Biochem. 375 265
  4. Nikolova-Karakashian (2021), Methods to Characterize Synthesis and Degradation of Sphingomyelin at the Plasma Membrane and Its Impact on Lipid Raft Dynamics; Methods Mol. Biol. 2187 113
CAS:
94885-02-6
Catalog Number:
10-5496
Activity:
Fluorescent probe for the Golgi apparatus
Chemical Names:
N-[7-(4-Nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine; N-(NBD-aminohexanoyl) D-erythro-sphingosine
Molecular Weight:
575.75
Molecular Formula:
C30H49N5O6
Solubility:
Soluble in DMSO (35 mg/mL)
Physical Properties:
Orange solid
Purity:
>98% TLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Stable for 2 years as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.
Shipping Code:
RT

Safety Data Sheet:

N/A

Product Data Sheet:

Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications. Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a $20 restocking/refund fee

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