Coelenterazine-h | Cell viability probe
NMR (Conforms)
Available Options
| Size : | Price | Quantity | |
|---|---|---|---|
| 1 mg | $150.00 | ||
| 5 mg | $600.00 |
Coelenterazine-h (50909-86-9) may be used as a substrate in gene reporter assays, live cell assays, and biochemical assays (ELISA, BRET etc). Renilla luciferase catalyzes coelenterazine oxidation by oxygen to produce light. Coelenterazine-h produces 10-fold higher light output than native coelenterazine. Coelenterazine-h also undergoes autoluminescence, which is enhanced by superoxide anion and peroxynitrite in cells.
References/Citations:
1) Shimomura et al. (1989), Semi-synthetic aequorins with improved sensitivity to Ca2+ ions; Biochem. J., 261 913
2) Nakajima-Shimada et al. (1991), Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system; Proc. Natl. Acad. Sci. USA, 88 6878
3) Inouye et al. (1992), Monitoring gene expression in Chinese hamster ovary cells using secreted apoaequorin; Anal. Biochem., 201 114
NMR (Conforms)
Safety Data Sheet:
Product Data Sheet:
Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications. Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a $20 restocking/refund fee
Coelenterazine-h (50909-86-9) may be used as a substrate in gene reporter assays, live cell assays, and biochemical assays (ELISA, BRET etc). Renilla luciferase catalyzes coelenterazine oxidation by oxygen to produce light. Coelenterazine-h produces 10-fold higher light output than native coelenterazine. Coelenterazine-h also undergoes autoluminescence, which is enhanced by superoxide anion and peroxynitrite in cells.
References/Citations:
1) Shimomura et al. (1989), Semi-synthetic aequorins with improved sensitivity to Ca2+ ions; Biochem. J., 261 913
2) Nakajima-Shimada et al. (1991), Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system; Proc. Natl. Acad. Sci. USA, 88 6878
3) Inouye et al. (1992), Monitoring gene expression in Chinese hamster ovary cells using secreted apoaequorin; Anal. Biochem., 201 114
Related products
-
4-MUG | ß-Galactosidase fluorogenic substrate
From $40.00CAS:6160-78-7Catalog Number:10-3879Activity:ß-Galactosidase fluorogenic substrateChemical Names:4-Methylumbelliferyl ß-D-galactopyranoside;
4-MU-b-D-Gal; MUgal; MUG; MUGAMolecular Weight:338.31Molecular Formula:C16H18O8Solubility:Soluble in DMSO (40 mg/ml)Physical Properties:White solidPurity:97% HPLC
NMR (Conforms)Storage Temperature:-20°CStability:Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.Shipping Code:RT -
Suc-Leu-Leu-Val-Tyr-AMC | Proteasome substrate
From $30.00CAS:94367-21-2Catalog Number:10-2971Activity:Proteasome substrateAlternate Names:Suc-LLVY-AMC, Proteasome Substrate III (Fluorogenic)Molecular Weight:763.88Molecular Formula:C40H53N5O10Solubility:Soluble in DMSO (up to 20 mg/ml), or in DMF (up to 10 mg/ml)Physical Properties:Lyophilized solidPurity:98% by HPLC
NMR (Conforms)Storage Temperature:-20°CStability:Stable for 1 year from date of purchase as supplied. Solutions in DMSO or DMF may be stored at -20°C for up to 1 month.Shipping Code:RT -
C6-NBD Ceramide | Fluorescent probe for the Golgi apparatus
From $100.00CAS:94885-02-6Catalog Number:10-5496Activity:Fluorescent probe for the Golgi apparatusChemical Names:N-[7-(4-Nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine; N-(NBD-aminohexanoyl) D-erythro-sphingosineMolecular Weight:575.75Molecular Formula:C30H49N5O6Solubility:Soluble in DMSO (35 mg/mL)Physical Properties:Orange solidPurity:>98% TLC
NMR (Conforms)Storage Temperature:-20°CStability:Stable for 2 years as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.Shipping Code:RT
Calculate the molar concentration, mass or volume in a solution.
Concentration × Volume × Molecular Weight = Mass
