Coelenterazine-h | Cell viability probe
NMR (Conforms)
Available Options
| Size : | Price | Quantity | |
|---|---|---|---|
| 1 mg | $150.00 | ||
| 5 mg | $600.00 |
Coelenterazine-h (50909-86-9) may be used as a substrate in gene reporter assays, live cell assays, and biochemical assays (ELISA, BRET etc). Renilla luciferase catalyzes coelenterazine oxidation by oxygen to produce light. Coelenterazine-h produces 10-fold higher light output than native coelenterazine. Coelenterazine-h also undergoes autoluminescence, which is enhanced by superoxide anion and peroxynitrite in cells.
References/Citations:
1) Shimomura et al. (1989), Semi-synthetic aequorins with improved sensitivity to Ca2+ ions; Biochem. J., 261 913
2) Nakajima-Shimada et al. (1991), Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system; Proc. Natl. Acad. Sci. USA, 88 6878
3) Inouye et al. (1992), Monitoring gene expression in Chinese hamster ovary cells using secreted apoaequorin; Anal. Biochem., 201 114
NMR (Conforms)
Safety Data Sheet:
Product Data Sheet:
Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications. Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a $20 restocking/refund fee
Coelenterazine-h (50909-86-9) may be used as a substrate in gene reporter assays, live cell assays, and biochemical assays (ELISA, BRET etc). Renilla luciferase catalyzes coelenterazine oxidation by oxygen to produce light. Coelenterazine-h produces 10-fold higher light output than native coelenterazine. Coelenterazine-h also undergoes autoluminescence, which is enhanced by superoxide anion and peroxynitrite in cells.
References/Citations:
1) Shimomura et al. (1989), Semi-synthetic aequorins with improved sensitivity to Ca2+ ions; Biochem. J., 261 913
2) Nakajima-Shimada et al. (1991), Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system; Proc. Natl. Acad. Sci. USA, 88 6878
3) Inouye et al. (1992), Monitoring gene expression in Chinese hamster ovary cells using secreted apoaequorin; Anal. Biochem., 201 114
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