DAPI | Fluorescent DNA stain
4′,6-Diamidino-2-phenylindole, dihydrochloride
NMR (Conforms)
Available Options
| Size: | Price | Quantity | |
|---|---|---|---|
| 5 mg | $45.00 | ||
| 25 mg | $150.00 |
DAPI (28718-90-3) is a fluorescent DNA stain for use on fixed cells for applications such as imaging, mitochondrial DNA detection1, or nuclear quantitation2. This dye, often used as a counterstain in immunofluorescence, binds strongly to AT rich runs of nucleic acid, causing its fluorescent emission to increase.3 Ex 358 nm; Em 461 nm (dsDNA), Em 500 (RNA).
References/Citations:
- Russell et al. (1975), A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses; Nature, 253 461
- Tarnowski et al. (1991), DAPI as a useful stain for nuclear quantitation; Biotech Histochem., 66 297
- Estandarte et al. (2016), The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes; Sci. Rep., 6 31417
4′,6-Diamidino-2-phenylindole, dihydrochloride
NMR (Conforms)
Safety Data Sheet:
Product Data Sheet:
Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications. Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a $20 restocking/refund fee
DAPI (28718-90-3) is a fluorescent DNA stain for use on fixed cells for applications such as imaging, mitochondrial DNA detection1, or nuclear quantitation2. This dye, often used as a counterstain in immunofluorescence, binds strongly to AT rich runs of nucleic acid, causing its fluorescent emission to increase.3 Ex 358 nm; Em 461 nm (dsDNA), Em 500 (RNA).
References/Citations:
- Russell et al. (1975), A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses; Nature, 253 461
- Tarnowski et al. (1991), DAPI as a useful stain for nuclear quantitation; Biotech Histochem., 66 297
- Estandarte et al. (2016), The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes; Sci. Rep., 6 31417
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