Nocodazole | Microtubule inhibitor

CAS:
31430-18-9
Catalog Number:
10-2387
Activity:
Microtubule inhibitor/CRISPR enhancer
Alternate Names:
R-17934
Chemical Name:
Methyl[5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl]carbamate
Molecular Weight:
301.32
Molecular Formula:
C14H11N3O3S
Solubility:
Soluble in DMSO (up to 10 mg/ml).
Physical Properties:
White or off-white solid
Purity:
98% by TLC
NMR (Conforms)
Storage Temperature:
RT (des.)
Stability:
Stable for 2 years as supplied. Solutions in DMSO may be stored at -20° for up to 2 months.
Shipping Code:
RT

Available Options

Size :PriceQuantity 
10 mg$50.00
50 mg$170.00

Nocodazole (31430-18-9) is an antimitotic agent that disrupts microtubules by binding to β tubulin and thereby inhibiting microtubule dynamics, disruption of mitotic spindle function, and fragmentation of the Golgi complex.1,2 Arrests cell cycle at G2/M phase. Stimulates the intrinsic GTPase activity of tubulin.3 Nocodazole activates the JNK/SAPK signaling pathway and induces apoptosis in a variety of cell lines.4 Increases Cas9-mediated editing frequencies5 and increased CRISPR-mediated HDR DNA repair6. Cell permeable.

References/Citations:

1) Jordan et al. (1992), Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis; J. Cell Science, 102 401
2) Storrie et al. (1998), Dynamics of the interphase mammalian Golgi complex as revealed through drugs producing reversible Golgi disassembly; Biochim. Biophys. Acta, 1404 127
3) Mejillano et al. (1996), Studies on the nocodazole-induced GTPase activity of tubulin; Arch. Biochem. Biophys., 336 130
4) Wang et al. (1998), Microtubule-interfering agents activate c-Jun N-terminal kinase/stress-activated protein kinase through both Ras and apoptosis signal-regulating kinase pathways; J. Biol. Chem., 273 4928
5) Lin et al. (2014), Enhanced homology-directed human genome engineering by controlled timing of CRSIPR/Cas9 delivery; eLife, 3 e04766
6) Li et al. (2023), Modulation of cell cycle increases CRISPR-mediated homology-directed DNA repair; Cell Biosci., 13 215

CAS:
31430-18-9
Catalog Number:
10-2387
Activity:
Microtubule inhibitor/CRISPR enhancer
Alternate Names:
R-17934
Chemical Name:
Methyl[5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl]carbamate
Molecular Weight:
301.32
Molecular Formula:
C14H11N3O3S
Solubility:
Soluble in DMSO (up to 10 mg/ml).
Physical Properties:
White or off-white solid
Purity:
98% by TLC
NMR (Conforms)
Storage Temperature:
RT (des.)
Stability:
Stable for 2 years as supplied. Solutions in DMSO may be stored at -20° for up to 2 months.
Shipping Code:
RT

Safety Data Sheet:

Product Data Sheet:

Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications. Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a $20 restocking/refund fee

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