Nocodazole (31430-18-9) is an antimitotic agent that disrupts microtubules by binding to β tubulin and thereby inhibiting microtubule dynamics, disruption of mitotic spindle function, and fragmentation of the Golgi complex1,2. Arrests cell cycle at G2/M phase. Stimulates the intrinsic GTPase activity of tubulin3. Nocodazole activates the JNK/SAPK signaling pathway and induces apoptosis in a variety of cell lines4. Cell permeable.
1) Jordan et al. (1992), Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis; J. Cell Science, 102 401
2) Storrie et al. (1998), Dynamics of the interphase mammalian Golgi complex as revealed through drugs producing reversible Golgi disassembly; Biochim. Biophys. Acta, 1404 127
3) Mejillano et al. (1996), Studies on the nocodazole-induced GTPase activity of tubulin; Arch. Biochem. Biophys., 336 130
4) Wang et al. (1998), Microtubule-interfering agents activate c-Jun N-terminal kinase/stress-activated protein kinase through both Ras and apoptosis signal-regulating kinase pathways; J. Biol. Chem., 273 4928