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Phosphatidylethanol | PLD assay standard

Phosphatidlyethanol is an endogenous metabolite of phosphatidylcholine. Phospholipase D converts phosphatidylcholine to phosphatidylethanol in the presence of ethanol. This reaction is the basis of a sensitive and selective assay for PLD activity in intact cells for which this product may be used as a chromatographic standard.

1) Kobayashi et al. (1987), Phosphatidylethanol formation via transphosphatidylation by rat brain synaptosomal phospholipase D; J. Neurochem., 48 1597
2) Liscovith et al. (1989), Phosphatidylethanol biosynthesis in ethanol-exposed NG108-15 neuroblastoma X glimoa hybrid cells. Evidence for activation of a phospholipase D phosphatidyl transferase activity by protein kinase C; J. Biol. Chem., 264 1450
3) Pai et al. (1988), Phospholipase D catalyzes phospholipid metabolism in chemotactic peptide-stimulated HL-60 granulocytes; J. Biol. Chem., 263 12472

Focus Biomolecules cell signaling reagent supplier, chemical structure of Phosphatidylethanol | PLD assay standard

Catalog#  10-1410

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Catalog Number:
PLD assay standard
Chemical Name:
Molecular Weight:
Molecular Formula:
Soluble in Chloroform (up to 10 mg/ml).
Physical Properties:
Viscous oil / waxy solid
98% by TLC
NMR (Conforms)
Storage Temperature:
Stable for 2 years as supplied. Phosphatidylethanol is subject to oxidation and must be protected from exposure to air. Solutions in chloroform may be stored at -20° for up to 3 months.
Shipping Code:
Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications.

Focus BiomoleculesPlymouth Meeting, PA USA1-855-FOCUS21

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