SAG | Smoothened agonist
NMR (Conforms)
Available Options
| Size : | Price | Quantity | |
|---|---|---|---|
| 1 mg | $35.00 | ||
| 5 mg | $95.00 |
SAG (364590-63-6) is a smoothened agonist which binds directly to SMO and can block SMO inhibition by cyclopamine (Cat.# 10-1082). The conformation of SMO is altered by SAG binding which results in the accumulation of SMO in cilia and activated gene transcription. SAG induces hedgehog pathway activation in a mouse cultured cell assay (EC50 = 3 nM) via binding to smoothened heptahelical bundle (KD = 59 nM).1,2 It should be noted that SAG inhibits hedgehog signaling at concentrations greater than 1 μM (EC50 for SMO activation is ~ 3 nM).1
References/Citations
1) Chen et al. (2002), Small molecule modulation of Smoothened activity; Proc. Natl. Acad. Sci. USA, 99 1407
2) Frank-Kamenestsky et al. (2002), Small molecule modulators of Hedgehog signaling: identifcation and characterization of Smoothened agonists and antagonists; J. Biol., 1 10
NMR (Conforms)
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Product Data Sheet:
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SAG (364590-63-6) is a smoothened agonist which binds directly to SMO and can block SMO inhibition by cyclopamine (Cat.# 10-1082). The conformation of SMO is altered by SAG binding which results in the accumulation of SMO in cilia and activated gene transcription. SAG induces hedgehog pathway activation in a mouse cultured cell assay (EC50 = 3 nM) via binding to smoothened heptahelical bundle (KD = 59 nM).1,2 It should be noted that SAG inhibits hedgehog signaling at concentrations greater than 1 μM (EC50 for SMO activation is ~ 3 nM).1
References/Citations
1) Chen et al. (2002), Small molecule modulation of Smoothened activity; Proc. Natl. Acad. Sci. USA, 99 1407
2) Frank-Kamenestsky et al. (2002), Small molecule modulators of Hedgehog signaling: identifcation and characterization of Smoothened agonists and antagonists; J. Biol., 1 10
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