2-NBD-Glucose (186689-07-6) is a fluorescent glucose uptake probe. May be used to monitor glucose uptake in live cells and as a cell viability indicator.1,2 Has been used to measure glucose uptake in cultured hippocampal astrocytes using confocal epifluorescence microscopy.3 NBD fluorescence typically displays excitation/emission maxima of 465/540 nm. Cell permeable.
1) Yoshioka et al. (1996), A novel fluorescent derivative of glucose applicable to the assessment of glucose uptake activity of Escherichia coli; Biochim. Biophys. Acta, 1289 5
2) Yamada et al. (2000), Measurement of glucose uptake and intracellular calcium concentration in single, living, pancreatic beta-cells; J. Biol. Chem., 275 22278
3) Loaiza et al. (2003), Glutamate triggers rapid glucose transport stimulation in astrocytes as evidenced by real-time confocal microscopy; J. Neurosci., 23 7337