Biotin isoxazole | Precipitates RNA Binding Proteins
NMR (Conforms)
Available Options
| Size: | Price | Quantity | |
|---|---|---|---|
| 1 mg | $100.00 | ||
| 5 mg | $360.00 |
Biotin-isoxazole (1377605-22-5) precipitates hundreds of RNA-binding proteins with significant overlap to the constituents of RNA granules.1,2 In one study roughly 580 nuclear proteins were identified as being precipitated by B-Isox including TAF15, RNA polymerase II (largest subunit), numerous subunits of the mediator complex as well as a variety of enzymes involved in epigenetic modification of histones.3 SR domains were found to be the determinant that facilitates B-Isox precipitation.4 Also allows for the fractionation / isolation of SG-associated proteins and RNA precipitates.5
References:
- Kato et al. (2012), Cell-free formation of rNA granules: low complexity sequence domains form dynamic fibers within hydrogels; Cell, 149 753
- Han et al. (2012), Cell-free formation of RNA granules: bound RNAs identify features and components of cellular assemblies; Cell, 149 768
- Kwon et al. (2013), Phosphorylation-regulated binding of RNA polymerase II to fibrous polymers of low-complexity domains; Cell, 155 1049
- Kwon et al. (2014), Poly-dipeptides encoded by the C9orf72 repeats bind nucleoli, impede RNA biogenesis, and kill cells; Science, 345 1139
- Panas et al. (2015), Methods for the characterization of stress granules in virus infected cells; Methods, 90 57
NMR (Conforms)
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Product Data Sheet:
Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications. Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a $20 restocking/refund fee
Biotin-isoxazole (1377605-22-5) precipitates hundreds of RNA-binding proteins with significant overlap to the constituents of RNA granules.1,2 In one study roughly 580 nuclear proteins were identified as being precipitated by B-Isox including TAF15, RNA polymerase II (largest subunit), numerous subunits of the mediator complex as well as a variety of enzymes involved in epigenetic modification of histones.3 SR domains were found to be the determinant that facilitates B-Isox precipitation.4 Also allows for the fractionation / isolation of SG-associated proteins and RNA precipitates.5
References:
- Kato et al. (2012), Cell-free formation of rNA granules: low complexity sequence domains form dynamic fibers within hydrogels; Cell, 149 753
- Han et al. (2012), Cell-free formation of RNA granules: bound RNAs identify features and components of cellular assemblies; Cell, 149 768
- Kwon et al. (2013), Phosphorylation-regulated binding of RNA polymerase II to fibrous polymers of low-complexity domains; Cell, 155 1049
- Kwon et al. (2014), Poly-dipeptides encoded by the C9orf72 repeats bind nucleoli, impede RNA biogenesis, and kill cells; Science, 345 1139
- Panas et al. (2015), Methods for the characterization of stress granules in virus infected cells; Methods, 90 57
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Focus Biomolecules • Plymouth Meeting, PA USA • 1-855-FOCUS21
Focus Biomolecules
Plymouth Meeting, PA USA
1-855-FOCUS21
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