CFSE | Cellular Proliferation Probe

CFSE Supplier | CAS 92557-80-7 | Focus Biomolecules

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CFSE (92557-80-7) is a fluorophore which can diffuse into cells and covalently react with intracellular proteins via its activated carboxy group and thus be retained in the cell for long periods of time.1 CFSE fluorescence  can be used to analyze cell proliferation2, track cell migration3 and in applications such as flow cytometry4. Excitation/emission max: 491 and 518 nm.

References/Citations:

  1. Breeuwer et al. (1996), A Novel Method for Continuous Determination of the Intracellular pH in Bacteria with the Internally Conjugated Fluorescent Probe 5 (and 6-)-Carboxyfluorescein Succinimidyl Ester; Appl. Environ. Microbiol., 62 178
  2. Lyons et al. (2000) Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution; J. Immunol. Methods, 243 147
  3. Parrish et al. (2009) Use of the intracellular fluorescent dye CFSE to Monitor lymphocyte migration and proliferation; Curr. Protoc. Immunol., Suppl. 84 4.9.1
  4. Kandg et al. (2023) Cytotoxicity of Human Hepatic Intrasinusoidal Gamma/Delta T Cells Depends of Phospho-antigen and NK Receptor Signaling; Anticancer Res., 43 63
5-FAM-SE, 92557-809-7, cellular proliferation probe, CFSE fluorescence, CFSE Supplier
Alternate Name:
5-Carboxyfluorescein NHS ester; 5-FAM-SE

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Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications. Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a $20 restocking/refund fee

CFSE (92557-80-7) is a fluorophore which can diffuse into cells and covalently react with intracellular proteins via its activated carboxy group and thus be retained in the cell for long periods of time.1 CFSE fluorescence  can be used to analyze cell proliferation2, track cell migration3 and in applications such as flow cytometry4. Excitation/emission max: 491 and 518 nm.

References/Citations:

  1. Breeuwer et al. (1996), A Novel Method for Continuous Determination of the Intracellular pH in Bacteria with the Internally Conjugated Fluorescent Probe 5 (and 6-)-Carboxyfluorescein Succinimidyl Ester; Appl. Environ. Microbiol., 62 178
  2. Lyons et al. (2000) Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution; J. Immunol. Methods, 243 147
  3. Parrish et al. (2009) Use of the intracellular fluorescent dye CFSE to Monitor lymphocyte migration and proliferation; Curr. Protoc. Immunol., Suppl. 84 4.9.1
  4. Kandg et al. (2023) Cytotoxicity of Human Hepatic Intrasinusoidal Gamma/Delta T Cells Depends of Phospho-antigen and NK Receptor Signaling; Anticancer Res., 43 63