Coelentarazine-h (50909-86-9) may be used as a substrate in gene reporter assays, live cell assays and biochemical assays (ELISA, BRET etc). Renilla luciferase catalyzes coelentarazine oxidation by oxygen to produce light. Coelentarazine-h produces 10-fold higher light output than native coelentarazine. Coelentarazine-h also undergoes autoluminescence which is enhanced by superoxide anion and peroxynitrite in cells.
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