Coelentarazine-h (50909-86-9) may be used as a substrate in gene reporter assays, live cell assays, and biochemical assays (ELISA, BRET etc). Renilla luciferase catalyzes coelentarazine oxidation by oxygen to produce light. Coelentarazine-h produces 10-fold higher light output than native coelentarazine. Coelentarazine-h also undergoes autoluminescence, which is enhanced by superoxide anion and peroxynitrite in cells.
1) Shimomura et al. (1989), Semi-synthetic aequorins with improved sensitivity to Ca2+ ions; Biochem. J., 261 913
2) Nakajima-Shimada et al. (1991), Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system; Proc. Natl. Acad. Sci. USA, 88 6878
3) Inouye et al. (1992), Monitoring gene expression in Chinese hamster ovary cells using secreted apoaequorin; Anal. Biochem., 201 114