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Product Details

Coelenterazine-h | Cell viability probe

Coelentarazine-h (50909-86-9) may be used as a substrate in gene reporter assays, live cell assays and biochemical assays (ELISA, BRET etc). Renilla luciferase catalyzes coelentarazine oxidation by oxygen to produce light. Coelentarazine-h produces 10-fold higher light output than native coelentarazine. Coelentarazine-h also undergoes autoluminescence which is enhanced by superoxide anion and peroxynitrite in cells.  

References/Citations

1) Shimomura et al. (1989), Semi-synthetic aequorins with improved sensitivity to Ca2+ ions; Biochem. J., 261 913
2) Nakajima-Shimada et al. (1991), Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system; Proc. Natl. Acad. Sci. USA, 88 6878
3) Inouye et al. (1992), Monitoring gene expression in Chinese hamster ovary cells using secreted apoaequorin; Anal. Biochem., 201 114

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Coelenterazine | 50909-86-9 | Structure | Supplier

Catalog#  10-1496-0001

$150.00
Quantity
Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a $20 restocking/refund fee.
 
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CAS:
50909-86-9
Catalog Number:
10-1496
Activity:
Cell viability probe
Chemical Name:
2,8-Dibenzyl-6-(4-hydroxyphenyl)imidazole[1,2-a]pyrazin-3(7H)-one
Alternate Names:
2-Deoxycoelenterazine
Molecular Weight:
407.46
Molecular Formula:
C26H21N3O2
Solubility:
Soluble in DMSO (up to 10 mg/ml) or in Methanol (up to 1 mg/ml)
Physical Properties:
Yellow solid
Purity:
97% by HPLC
NMR (Conforms)
Storage Temperature:
-20°C (des.)
Stability:
Stable for 1 year as supplied. Solutions in DMSO or methanol may be stored at -20°C for up to 1 month.
Shipping Code:
RT
Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications.

Focus BiomoleculesPlymouth Meeting, PA USA1-855-FOCUS21

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