E-64d | Cysteine protease inhibitor

Cell-permeable derivative of E-64c, an inhibitor of calpain and other cysteine proteases, such as papain, cathepsin B and cathepsin L.^1,2 Addition to cell cultures should be done in serum-free media as esterases in serum will cleave the ethyl ester and reduce cell permeability. Typical working concentration is 0.5-10 µg/ml. Inhibits degradation of autophagic cargo inside autolysosomes.³

References/Citations
1) McGowan et al. (1989), Inhibition of calpain in intact platelets by the thiol protease inhibitor E-64d; Biochem. Biophys. Res. Commun., 158 432
2) Wilcox and Mason (1992), Inhibition of cysteine proteinases in lysosomes and whole cells; Biochem. J., 285 495
3) Mizushima et al. (2010), Methods in mammalian autophagy research; Cell, 140 313