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Product Details

E-64d | Cysteine protease inhibitor

Cell-permeable derivative of E-64c, an inhibitor of calpain and other cysteine proteases, such as papain, cathepsin B and cathepsin L.1,2 Addition to cell cultures should be done in serum-free media as esterases in serum will cleave the ethyl ester and reduce cell permeability. Typical working concentration is 0.5-10 µg/ml. Inhibits degradation of autophagic cargo inside autolysosomes.3

References/Citations
1) McGowan et al. (1989), Inhibition of calpain in intact platelets by the thiol protease inhibitor E-64d; Biochem. Biophys. Res. Commun., 158 432
2) Wilcox and Mason (1992), Inhibition of cysteine proteinases in lysosomes and whole cells; Biochem. J., 285 495
3) Mizushima et al. (2010), Methods in mammalian autophagy research; Cell, 140 313

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Catalog#  10-1347-0001

$45.00
Quantity
Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a $20 restocking/refund fee.
 
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CAS:
88321-09-9
Catalog Number:
10-1347
Activity:
Cysteine protease inhibitor
Chemical Name:
2S,3S-trans-(Ethoxycarbonyloxirane-2-carbonyl)-L-leucine-(3-methylbutyl)amide
Molecular Weight:
342.43
Molecular Formula:
C17H30N2O5
Solubility:
Soluble in DMSO (up to 20 mg/ml) or in Ethanol (up to 10 mg/ml).
Physical Properties:
White or off-white solid
Purity:
98% by HPLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Stable for 1 year as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month.
Shipping Code:
RT
Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications.

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