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Product Details

JC-1 | Mitochondrial fluorescent probe

JC-1 (CAS 3520-43-2) is a dual emission, potential-sensitive, carbocyanine probe which can be used to measure mitochondrial membrane potential.  When excited at 490 nm the monomer exhibits an emission maximum at 527 nm and J-aggregates at 590 nm.1,2  JC-1 changes color reversibly from green to red with increasing mitochondrial membrane potential.3  May be used for isolation of live cells expressing high multidrug resistance transport activity.4  A widely used probe for determination of mitchondrial membrane potential.5


1) Reers et al. (1991), J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential; Biochemistry, 30 4480
2) Smiley et al. (1991), Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1; Proc. Natl. Acad. Sci. USA, 88 3671
3) Lisa et al. (1995), Mitochondrial membrane potential in single living adult rat cardiac myocytes exposed to anoxia or metabolic inhibition; J. Physiol., 486 1
4) Wolosin et al. (2017), Application of JC1 for non-toxic isolation od cells with MDR transporter activity by flow cytometry; PLoS One, 12(4) e0174905
5) Wang et al. (2017), A novel cytoprotective peptide protects mesenchymal stem cells against mitochondrial dysfunction and apoptosis induced by starvation via Nrf2/Sirt3/FoxO3a pathway; J. Transl. Med., 15 33

Focus Biomolecules reagent supplier, chemical structure of JC-1 | Mitochondrial membrane potential probe | CAS 3520-43-2

Catalog#  10-1634

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Catalog Number:
Mitochondrial membrane potential probe
Chemical Name:
5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide
Molecular Weight:
Molecular Formula:
Soluble in DMSO (up to 15 mg/ml) or in Water (up to 50 mg/ml)
Physical Properties:
Brown solid
98% by HPLC
NMR (Conforms)
Storage Temperature:
-20°C (des.)
Store as supplied desiccated at -20°C for up to 2 years from the date of purchase. Solutions in DMSO or distilled water may be stored at 4°C for up to 1 year. DO NOT FREEZE/THAW SOLUTIONS. Sterilize solutions only by filtration, not by autoclaving
Shipping Code:
Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications.

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