MitoBloCK-6 inhibits redox-regulated protein translocation into mitochondria. It attenuates the import of Erv1 substrates by inhibition of Erv1 oxidase activity (the import of Tim23 and AAC was decreased by ~50% at 20 μM). MitoBloCK-6 elucidated an unexpected role for Erv1 in the carrier import pathway, namely transferring substrates from the translocase of the outer membrane complex into the small Tim complexes. It impaired cardiac development in zebrafish embryos and induced apoptosis via the release of cytochrome c in human embryonic stem cells but not differentiated cells1.
1) Dabir et al. (2013), A small molecule inhibitor of redox-regulated protein translocation into mitochondria; Dev. Cell, 25 81