Propidium Iodide | Fluorescent stain for dead/dying cells

CAS:
25535-16-4
Catalog Number:
10-5165
Activity:
Fluorescent stain for dead/dying cells
Chemical Names:
3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
Molecular Weight:
668.39
Molecular Formula:
C27H34I2N4
Solubility:
Soluble in DMSO (2.5 mg/ml); water (3 mg/ml)
Physical Properties:
Red solid
Purity:
>95% HPLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Stable for 2 years from date of purchase as supplied. Solutions in DMSO or water may be stored at -20°C for up to 1 month.
Shipping Code:
RT

Available Options

Size:PriceQuantity 
10 mg$40.00
50 mg$90.00
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Propidium iodide (25535-16-4) is a red fluorescent DNA stain.1,2 Membrane impermeable to live cells but permeable to dead or damaged cells and thus may be used for identifying cell viability or as a counterstain.3,4 Binds to DNA by intercalation and is suitable for fluorescence microscopy and flow cytometry.2 Ex.: 488-535 nm and Em.: 617 nm.

References/Citations:

  1. Coder et al. (1997), Assessment of cell viability; Curr. Protoc. Cytom., Suppl. 15
  2. Edwards et al. (2007), High-throughput cytotoxicity screening by propidium iodide staining; Curr. Protoc. Cytom., Unit 9.24
  3. Sawai and Domae (2011), Discrimination between primary necrosis and apoptosis by necrostatin-1 in Annexin V-positive/propidium iodide-negative cells; Biochem. Biophys. Res. Commun., 411 569
  4. Pizato et al. (2018), Omega-3 docosahexaenoic acid induces pyroptosis cell death in triple-negative breast cancer cells; Sci. Rep., 8 1952
CAS:
25535-16-4
Catalog Number:
10-5165
Activity:
Fluorescent stain for dead/dying cells
Chemical Names:
3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
Molecular Weight:
668.39
Molecular Formula:
C27H34I2N4
Solubility:
Soluble in DMSO (2.5 mg/ml); water (3 mg/ml)
Physical Properties:
Red solid
Purity:
>95% HPLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Stable for 2 years from date of purchase as supplied. Solutions in DMSO or water may be stored at -20°C for up to 1 month.
Shipping Code:
RT

Safety Data Sheet:

N/A

Product Data Sheet:

N/A

Materials provided by Focus Biomolecules are for laboratory research use only and are not intended for human or veterinary applications. Please note that we do not sell to individuals and that all orders placed by non-research organizations will incur a $20 restocking/refund fee

Propidium iodide (25535-16-4) is a red fluorescent DNA stain.1,2 Membrane impermeable to live cells but permeable to dead or damaged cells and thus may be used for identifying cell viability or as a counterstain.3,4 Binds to DNA by intercalation and is suitable for fluorescence microscopy and flow cytometry.2 Ex.: 488-535 nm and Em.: 617 nm.

References/Citations:

  1. Coder et al. (1997), Assessment of cell viability; Curr. Protoc. Cytom., Suppl. 15
  2. Edwards et al. (2007), High-throughput cytotoxicity screening by propidium iodide staining; Curr. Protoc. Cytom., Unit 9.24
  3. Sawai and Domae (2011), Discrimination between primary necrosis and apoptosis by necrostatin-1 in Annexin V-positive/propidium iodide-negative cells; Biochem. Biophys. Res. Commun., 411 569
  4. Pizato et al. (2018), Omega-3 docosahexaenoic acid induces pyroptosis cell death in triple-negative breast cancer cells; Sci. Rep., 8 1952

Focus Biomolecules • Plymouth Meeting, PA USA • 1-855-FOCUS21

Focus Biomolecules
Plymouth Meeting, PA USA
1-855-FOCUS21

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