SR1 (1227633-49-9) extends the pluripotency of hematopoietic stem cells by antagonizing the arylhydrocarbon receptor (AhR), IC50 = 127 nM.1 The effect is dose-dependent and reversible. Displays a biphasic effect on CD34-negative cells with low does (1 µM) enhancing proliferation and high does (>5 µM) repressing proliferation.2 May be used to generate progenitor T cells3 from HSPCs as well as NK cells for immuno-oncology studies4.
1) Boitano et al. (2010), Aryl hydrocarbon receptor antagonists promote the expansion of human hematopoietic stem cells; Science, 329 1354
2) Tao et al. (2017), StemRegenin 1 selectively promotes expansion of Multipotent Hematopoietic Progenitors derived from Human Embryonic Stem Cells; J. Stem Cells Regen. Med., 13 75
3) Singh et al. (2019), Generation and function of progenitor T cells from stemRegenin-1-expanded CD34+ human hematopoietic progenitor cells; Blood Adv, 3 2934
4) Hoogstad-van Evert et al. (2017), Umbilical cord blood CD34+ progenitor-derived NK cells efficiently kill ovarian cancer spheroids and intraperitoneal tumors in NOD/SCID/IL2Rgnull mice; Oncoimmunology, 6(8) e1320630