Dansylcadaverine | Autophagic vacuole dye

Dansylcadaverine (10121-91-2) preferentially accumulates in autophagic vacuoles due to a combination of ion trapping and specific interactions with membrane lipids making it a very useful probe for monitoring autophagy.^1,2 Inhibits EGF internalization.³ Dansylcadaverine competes with certain trans-aminases and can be used with antibody/affinity procedures to isolate resulting conjugates.⁴ Abs: 335 nm, Em: 510 nm

References/Citations

1) Munafo and Colombo (2001), A novel assay to study autophagy: regulation of autophagosome vacuole size by amino acid deprivation; Int. J. Cell Sci., 114 3619
2) Jiang et al. (2012), Targeting androgen receptor leads to suppression of prostate cancer via induction of autophagy; J. Urol., 188 1361
3) Haigler et al. (1980), Dansylcadaverine inhibits internalization of 125I-epidermal growth factor in BALB 3T3 cells; J. Biol. Chem., 255 1239
4) Murthy et al. (1994), Residue Gln-30 of human erythrocyte anion transporter is a prime site for reaction with intrinsic transglutaminase; J. Biol. Chem., 269 22907