Phosphoramidon (119942-99-3) inhibits neutral endopeptidase 24.11 (NEP; Neprilysin; EC 220.127.116.11), Ki=2 nM.1 Also inhibits neprilysin 2 (Ki~2 nM).2 Also inhibits endothelin-converting enzyme (IC50 = 0.68 μM).3 Induces a dramatic increase in amyloid-beta levels in rodents.4
1) Stephenson and Kenny et al. (1987), The hydrolysis of alpha-human atrial natriuretic peptide by pig kidney microvillar membranes is initiated by endopeptidase-24.11.; Biochem. J., 243 183.
2) Rose et al. (2002), Cell-specific activity of neprilysin 2 isoforms and enzymic specificity compared with neprilysin; Biochem. J., 363 697
3) Ahn et al. (1998), Soluble human endothelin-converting enzyme-1: expression, purification, and demonstration of pronounced pH sensitivity; Arch. Biochem. Biphys., 359 258
4) Marr and Hafez (2014), Amyloid-beta and Alzheimer’s disease: the role of neprilysin-2 in amyloid-beta clearance; Front. Aging Neurosci., 6 187